When you industrially produce or study the properties of natural or recombinant proteins, their quality must be very high. Protein purification can be implemented by different techniques, all of which must consider three important factors in the purification protocol: purity, the desired amount of protein and the associated purification costs. In the case of enzymes, preserving their best activity during a process is a variable that you will need to consider.
The costs of purifying molecules
The important aspect of costs varies depending on the techniques used, the materials used, the quantities. Very often, these costs can be a limit in the preparation on a large scale, therefore it is essential to make a careful prior assessment, considering the best relationship between costs and benefits.
Collaborating with BiCT you will be able to use the most advanced technologies of purification of biomolecules, choosing according to your needs and objectives. You will optimize entire purification processes, or part of them. For example, in the specific case of chromatography, our team of professionals will develop the complete process and can also support you in supplying resin on an industrial scale. This will optimize your costs and greatly facilitate the passage of scale.
The purity you need
The degree of purity is closely related to the final objectives. If you want to obtain an enzyme preparation for industrial purposes, taking into account the areas of application, a very high degree of purity is often not necessary. In case you want to carry out biochemical studies, we will take care of achieving a higher degree of purity.
In addition, if you have structural studies as your goal, purity and homogeneity must be maximum. In this case, we will work on purities close to 100%.
In the case of pharmaceutical use, purity is even more important, a variable that can make the difference with your competitors, also with the high level you will ensure the safety of users and compliance with regulatory aspects.
The amount of protein for your needs
The final step of the productive scale will be a criterion by which we will decide with you and for you the choice of the purification technique.
In the case of large quantities of material, we will use techniques such as selective precipitation or chromatography with resins that can perform many high-performance cycles.
We will apply affinity chromatography in order to obtain particularly pure molecules. This technique, for example, is particularly used for molecules for pharmaceutical use, such as monoclonal antibodies.